Part:BBa_K678049

pJEJAM1

pJEJAM1 is a plasmid intended for transient transfection of mammalian cells. pJEJAM1 consists of the strong constitutive cytomegalovirus (CMV) promoter, green fluorescence protein (GFP), the BGH polyA, the hygR marker cassette, an ori for propagation in E.coli and ampR resistance cassette (Figure 1).

DTU-Denmark-2 2011 Figure 1: Plasmid map of pJEJAM1, the figure is not drawn to scale.

Expression of pJEJAM1 in the mammalian cell line U-2 OS results in a homogenous distribution of GFP in the cells (Figure 2 and Figure 3).

DTU-Denmark-2 2011 Figure 2: U-2 OS cells transiently transfected with pJEJAM1 expressing GFP. The white bar has a length of 20μm.

DTU-Denmark-2 2011 Figure 3: U-2 OS cells transiently transfected with pJEJAM1 expressing GFP. The white bar has a length of 20μm.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2216
  Illegal PstI site found at 2306
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2216
  Illegal PstI site found at 2306
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2216
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2216
  Illegal PstI site found at 2306
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2216
  Illegal PstI site found at 2306
  Illegal NgoMIV site found at 3071
  
• 1000
  COMPATIBLE WITH RFC[1000]