DNA
Part:BBa_K524000:Design
Designed by: HO Yuan Heng Trevor Group: iGEM11_HKUST (2011-09-15)
Heat sensitive origin of replication (oriR101 & repA101-ts)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1709
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To make this part compatible with Biobrick standard assembly protocols, overlapping PCR was used to mutate a SpeI cut site originally present inside the construct to render it nonfunctional.
Source
From the plasmid pKD46 maintained inside the E. coli strain BW25113, courtesy of E.coli Genetic Resources at Yale CGSC, The Coli Genetic Stock Center.