Composite
Part:BBa_K566017:Design
Designed by: Daniel Rodriguez Group: iGEM11_UANL_Mty-Mexico (2011-09-26)
pRM434-RFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 681
Illegal AgeI site found at 793 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Search for illegal sites was performed, sites were localized and deleted.
Source
Synthesized DNA.
References
1. Dodd BI, Perkins AJ, Tsemitsidis D, Egan BJ (2001) Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny Gene Dev 15:3013–3022.
2. Révet B, von Wilcken-Bergmann B, Bessert H, Barker A, Müller-Hill B (1999) Four dimers of repressor bound to two suitably spaced pairs of operators form octamers and DNA loops over large distances. Curr Biol 9:151–154.