Composite
Part:BBa_K575026
Designed by: Kristin Palarz Group: iGEM11_Northwestern (2011-09-22)
Revision as of 01:13, 17 October 2018 by IsaacApplebaum (Talk | contribs)
LasR/PAI1 Inducible Promoter + RBS (B0030) + RFP, Constitutive Promoter + RBS (B0030) + LasR
This construct contains a LasR/PaI1 (3-oxo-C12-HSL) inducible promoter with an RBS and an RFP reporter. There is also constitutive transcription of the LasR receptor protein. Thus, the promoter will activate and fluorescence will be observed in the presence of PAI1.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 58
Illegal NheI site found at 907
Illegal NheI site found at 930 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1286
Illegal AgeI site found at 741
Illegal AgeI site found at 853
Illegal AgeI site found at 1483 - 1000COMPATIBLE WITH RFC[1000]
[http://2018.igem.org/Team:RMHS_Maryland Team RMHS_Maryland 2018] contributed to the characterization of this part by demonstrating that the part produces RFP expression when induced by AI-1.
(--ishayardi 20:24, 16 October 2018 (UTC))
Team RMHS_Maryland 2018: Characterization of AI-1 Induced Fluorescence in E. Coli Escherichia coli for use in co-culture
[edit]
Categories
Parameters
n/a | LasP/30/R + CP/30/LasR |