Plasmid

Part:BBa_K530028

Designed by: Daniel Bibl   Group: iGEM11_Johns_Hopkins   (2011-09-24)
Revision as of 01:41, 25 September 2011 by Dbibl1 (Talk | contribs)

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pRSBB403 HIS3 Integrating Yeast Shuttle Vector

Yeast shuttle vector for propagation in E. coli and transformation into S. cerevisiae. Contains an Ampicillin resistance marker for E. coli and HIS3 selectable marker for S. cerevisiae. Fully compatible for use with Standard Assembly. Contains EcoRI, XbaI, SpeI, and PstI in the multiple cloning site, in that order. This is chromosomal integrating vector.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1008
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 899
    Illegal BglII site found at 959
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1748
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3428
    Illegal SapI site found at 2345


Plasmid Map

PRSBB403.png

[edit]
Categories
Parameters
None