Plasmid

Part:BBa_K530034

Designed by: Daniel Bibl   Group: iGEM11_Johns_Hopkins   (2011-09-24)
Revision as of 00:51, 25 September 2011 by Dbibl1 (Talk | contribs)

pRSBB415 LEU2 CEN/ARS Yeast Shuttle Vector Yeast shuttle vector for propagation in E. coli and transformation into S. cerevisiae. Contains an Ampicillin resistance marker for E. coli and LEU2 selectable marker for S. cerevisiae. Fully compatible for use with Standard Assembly. Contains EcoRI, XbaI, SpeI, and PstI in the multiple cloning site, in that order. This vector is a CEN/ARS episomal yeast vector.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2799
    Illegal AgeI site found at 1503
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4479
    Illegal SapI site found at 3396
    Illegal SapI site found at 5500


Plasmid Map

PRS415.png

[edit]
Categories
Parameters
None