Coding
ADC-AAR
Part:BBa_K590052
Designed by: Austin Moon, Casey Ager, Ingrid Swanson, Seth Sagulo Group: iGEM11_Washington (2011-09-15)
Aldehyde Decarbonylase to Acyl-ACP Reductase (ADC-AAR) Fusion
This part encodes a direct gene fusion product of the two enzymes, Aldehyde Decarbonylase (ADC) and Acyl-ACP Reductase(AAR), in a linear configuration with a glycine-serine linker for co-localization. For more information, refer to [http://2011.igem.org/Team:Washington University of Washington 2011 iGEM Team wiki]
Usage and Biology
We expected this will optimize alkane production developed by the 2011 University of Washington iGEM Team, by bringing ADC and AAR close together. Contrary to what was expected, our gas chromatography-mass spectroscopy (GC-MS) data demonstrated that direct fusion has a negative effect on alkane production.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |