Regulatory

Part:BBa_K575023:Design

Designed by: Michael Vincent Sherer   Group: iGEM11_Northwestern   (2011-09-22)
Revision as of 21:28, 24 September 2011 by Mvs678 (Talk | contribs) (→‎Design Notes)

Genomic RhlR/PAI2 Inducible Promoter (short)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 304
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 357
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is unusually long for a promoter because of the primers we used to extract it. We do not know exactly where the promoter sequence lies within the part.

Source

Upstream Regulatory Region of the RhlAB operon from PA-01.

References