Part:BBa_K606040:Experience

The Hyperspank promoter was designed to be compatible for B. subtilis. It is shorter than the classic one, but is also recognised by E. coli polymerases.

We characterised it with an RFP expression system (PHs-RBS-RFP), into TURBO cells that express LacI to allow the promoter to be the less leacky possible. This system is inducible by IPTG.

Without IPTG

E.coli containing Hyperspank+RBS+RFP - IPTG at 37°C
-IPTG E.coli at 37°C (rfp image)	-IPTG E.coli at 37°C (trans image)
-IPTG E.coli at 37°C (rfp image)	-IPTG E.coli at 37°C (trans image)

With IPTG :

E.coli containing Hyperspank+RBS+RFP + IPTG at 37°C
+IPTG E.coli at 37°C (rfp image)	+IPTG E.coli at 37°C (trans image)
+IPTG E.coli at 37°C (rfp image)	+IPTG E.coli at 37°C (trans image)

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This part was used as a part of the Bee subtilis, iGEM METU 2013 project. We simulated the production of RFP under two different IPTG concentrations to observe its basal and maximum transcription rates.

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