Composite

Part:BBa_K575036

Designed by: Kristin Palarz   Group: iGEM11_Northwestern   (2011-09-22)
Revision as of 18:26, 24 September 2011 by Vc11 (Talk | contribs)

RhlR/PAI2 Inducible Promoter + RBS (B0034) + GFP + Constitutive Promoter + RBS (B0030) + RhlR

Promoter activated by RhlR/C4-HSL complex. Activation leads to GFP expression. Continuous expression of RhlR.Rhlp-34-gfp cp-30-rhlr.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 814
    Illegal NheI site found at 837
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1111
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1586
    Illegal BsaI.rc site found at 723


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Categories
Parameters
None