Part:BBa_K606025

YFP:tetR recombinant fusion protein

YFP:tetR is a recombinant fusion protein. It is composed of Yellow Fluorescent Protein (YFP) and Tetracycline Repressor Protein (tetR) that can bind to the tet operator sequence (tetO). Using the tetO array (BBa_K606026) composed of a 3,7kb tetO sequence, we can concentrate YFP:tetR in a few loci and increase the fluorescence sensibility.

Origins of YFP:tetR and tetO Array come from François-Xavier Barre, Andrew Wright and Dave Lane (Kinetics of plasmid segregation, Molecular Microbiology, 2004)

Usage and Biology

The combination of both parts K606025 and K606026 can be use to concentrate a fluorescence signal and make a smaller nmber of fluorescent proteins visible under the microscope. See the experiments page for characterization and picture of what can be seen under the microscope.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

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• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]