Coding

Part:BBa_K515007

Designed by: Atipat Patharagulpong   Group: iGEM11_Imperial_College_London   (2011-09-12)
Revision as of 02:47, 22 September 2011 by RebekkaBauer (Talk | contribs)

Dendra2


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


This BioBrick has been sequence verified.

Description

This part consists of the Dendra2 coding sequence codon optimised for Escherichia coli. It is part of the composite BioBrick BBa_K515107. This sequence codes for the photoconvertible protein Dendra2, capable of being irreversibly photoconverted from excitation at 486nm and emission at 505nm wavelength to 558nm excitation and 575nm emission wavelength. Photoconversion can occur using two wavelengths 488 and 405nm[1].

References

[1] Gurskaya N et al. (2006) Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light. Nature Biotechnology 24: 461-465.

[edit]
Categories
//cds
Parameters
chassisE. coli DH5α
device_typeFluorescent reporter
emission505 nm and 575 nm
excitation486 nm and 558 nm
proteinK515007
resistanceChloramphenicol