Coding

Part:BBa_K676006:Design

Designed by: Ejaj Intisar   Group: iGEM11_UCL_London   (2011-09-17)
Revision as of 03:20, 2 October 2011 by Eintisar (Talk | contribs) (→‎Design Notes)

T5 Phage Exonuclease


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 600
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The exonuclease is coded for by the D15 gene in the T5 bacteriophage genome. The gene was cloned out by PCR using designed primers. After cloning, site-directed mutagenesis was carried out to remove the XbaI restriction site loacted at the 225th to 230th nucleotide in the DNA sequence.


Primer Design for Exonuclease.jpg

Source

T5 Bacteriophage

References