Coding

Part:BBa_K515007

Designed by: Atipat Patharagulpong   Group: iGEM11_Imperial_College_London   (2011-09-12)
Revision as of 01:46, 22 September 2011 by Nk2909 (Talk | contribs)

Dendra2


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Description

This part consists of coding sequence codon optimised for Escherichia coli. It is part of the composite biobrick BBa_K515107. This sequence codes for photoconvertible protein Dendra, capable of being irreversibly photoconverted by single-photon stimulation from excitation at 486nm and emission at 505nm wavelength to 558nm excitation and 575nm emission wavelength. Photoconversion can occur using two wavelengths 488 and 405nm[1].

Reference

[1] Gurskaya N et al. (2006) Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light. Nature Biotechnology 24: 461-465.

This BioBrick has been sequence verified.

[edit]
Categories
//cds
Parameters
chassisE. coli DH5α
device_typeFluorescent reporter
emission505 nm and 575 nm
excitation486 nm and 558 nm
proteinK515007
resistanceChloramphenicol