DNA

Part:BBa_K676011:Design

Designed by: Meng Li   Group: iGEM11_UCL_London   (2011-09-19)
Revision as of 01:37, 22 September 2011 by Meng (Talk | contribs) (→‎References)

Gyrase Binding Site from pSC101


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR and then 2 parts ligation The GBS in pSC101 plasmid (285 bp) from 4580 to 4864: Entire pSC101 Sequence - 9263 bp

CAGTCTGAATGACCTGTCACGGGATAATCCGAAGTGGTCAGACTGGAAAAT CAGAGGGCAGGAACTGCTGAACAGCAAAAAGTCAGATAGCACCACATAGCA GACCCGCCATAAAACGCCCTGAGAAGCCCGTGACGGGCTTTTCTTGTATTA TGGGTAGTTTCCTTGCATGAATCCATAAAAGGCGCCTGTAGTGCCATTTAC CCCCATTCACTGCCAGAGCCGTGAGCGCAGCGAACTGAATGTCACGAAAAA GACAGCGACTCAGGTGCCTGATGGTCGGAG

Source

pSC101 plasmid

References

Mark Oram , Alison J. Howells, Anthony Maxwell and Martin L . Pato (2003)A biochemical analysis of the interaction of DNA gyrase with the bacteriophage Mu , pSC101 and pBR322 Strong gyrase Sites ; the role of DNA sequence in modulating gyrase supercoiling and biological activity ; Molecular Microbiology 50(1).333-347