Regulatory

Part:BBa_K091104:Experience

Designed by: Robert Cool   Group: iGEM08_Davidson-Missouri_Western   (2008-06-19)
Revision as of 08:52, 27 September 2011 by Vinchhi (Talk | contribs) (→‎User Reviews)

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Applications of BBa_K091104

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[http://2011.igem.org/Team:KULeuven K.U.Leuven iGEM 2011 Team]

Characterization by K.U.Leuven 2011 iGEM Team

We transformed Bba_K091104 (=pLac/Mnt hybrid) in DH5α-cells, followed by a minipreparation. After this we did a restriction digest with EcoRI and SpeI. Since the pSB1A2 vector is 2079bp and the Lac/Mnt hybrid promoter should be 87bp, we also expect a fragment of 87bp, one of 2079 and one of 2166bp. However, we see a fragment of 1000bp and one of ±2000bp.

Restriction 1807.jpg


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