Generator

Part:BBa_K638403

Designed by: Joseph Harvey   Group: iGEM11_Cambridge   (2011-09-20)
Revision as of 23:24, 21 September 2011 by Cat (Talk | contribs)

Arabinose inducible TorA-Reflectin A1-sfGFP generator

This construct is Reflectin A1 protein with a TorA signal peptide on the N terminus, and superfolder GFP (sfGFP) fused at the C-terminus. The reflectin A1 sequence is codon-optimized for E coli. The TorA signal peptide has been shown to allow export of GFP via the twin arginine pathway. The pBAD promoter is inducible with arabinose.

Usage and Biology

Safety

The protein coding sequence for reflectin originally came from cells of an edible squid. There have been no reported safety issues for reflectins, so we do not anticipate the need for extra precautions when using this BioBrick part. See our [http://2011.igem.org/Team:Cambridge/Safety safety page] for more information.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1369
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 2437


[edit]
Categories
Parameters
n/aArabinose inducible TorA-Reflectin A1-sfGFP generator