Part:BBa_K525710:Design

DNA ligase from Escherichia coli (LigA) with PT7, RBS and His-tag

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1449
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 1663
  

Design Notes

This is the complete sequence of NAD[^{+]-dependent DNA ligase with PT7, RBS and His-tag}

Source

PCR on the Escherichia coli TOP 10 DNA cloned into BioBrick Vectors

   Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix in the rev primer.
       fwd: 
       rev:

===References===