Tag

Part:BBa_K208005:Experience

Designed by: USU iGEM 2009   Group: iGEM09_Utah_State   (2009-10-12)
Revision as of 22:18, 21 September 2011 by Nicole (Talk | contribs) (→‎Applications of BBa_K208005)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K208005

Done by iGEM11 Potsdam_Bioware

The TorA signal sequence was used in combination with the beta lactamase (BBa_I757010) as a new protease activity detector device. The BioBrick parts BBa_K627012 and BBa_K627013 consist of the TorA signal sequence followed by a short peptide sequence used as cleavage site for different proteases (TEV (BBa_K627008-BBa_K627010) and 14_3C (BBa_K627011)) and ends with the beta lactamase. When E.coli XL1 blue where transformed with our construct, the cells were able to survive at ampicillin concentrations up to 400 µg/ml, when incubated on agar plates over night at 37°C, or up to 300 µg/ml ampicillin, when incubated over night at 30 °C, which indicates a high export rate via the TAT pathway.

User Reviews

UNIQ8c2f3165eb070f5e-partinfo-00000000-QINU UNIQ8c2f3165eb070f5e-partinfo-00000001-QINU