Part:BBa_K607003

Prm_GFP-LVA

GFP with LVA-tag under transcriptional control of a modified Prm(PcI) promoter. The Prm promotor is induced by cI. For more information on cI, see: https://parts.igem.org/wiki/index.php?title=Part:BBa_K607001
Activation of PRM involves an interaction between an acidic patch on the surface of the helix–turn–helix motif of cI protein and a target region near the C terminus of the σ70 subunit of Escherichia coli RNA polymerase. The GFP protein contains an LVA tag, so it will be degraded faster by proteolytic enzymes compared to GFP proteins without LVA tag.

References
Changing the mechanism of transcriptional activation by phage λrepressor. Mei Li,* W. R. McClure,† and Miriam M. Susskind. Proc Natl Acad Sci U S A. 1997.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 752