Composite

Part:BBa_K568000

Designed by: Team TU Munich 2011   Group: iGEM11_TU_Munich   (2011-09-01)
Revision as of 17:27, 21 September 2011 by Thorsten mueller (Talk | contribs) (→‎Usage and Biology)

Red light sensor

Single red light inducible construct without downstream genes. This part can be cloned upstream of any desired gene product.


Usage and Biology

Red light induces the autophosphorylation at the cytosolic site of cph8. This leads to phosphorylation of OmpR which subsequently binds to OmpC promotor and enables transcription of a following gene. This part needs an EnvZ deficient strain such as CP919 to function properly, as EnvZ pathway is supposedly also responsible for sensing of aspartate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 644
    Illegal XhoI site found at 1786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 971
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

n/aRed light sensor

Induction with red light at 705 nm.

Since OmpR is expressed phosphorylated and therefore gene expression is induced in the dark, red light signalling can be shut down with 650 nm.[1]

[edit]
Categories
Parameters
n/aRed light sensor