Composite

Part:BBa_K568000:Design

Designed by: Team TU Munich 2011   Group: iGEM11_TU_Munich   (2011-09-01)
Revision as of 14:33, 21 September 2011 by Thorsten mueller (Talk | contribs)

Red light sensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 644
    Illegal XhoI site found at 1786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 971
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR product on relevant part of K322127


Sequence

Sequence of first 1000 bp of PCR-Product shows one possible silent mutation in PcyA (Phycocyanobilin:ferredoxin oxidoreductase) gen on Position 45 T->C. Resulting triplet is CCC instead of CCT, which both code for proline.


References

[1] G A Gambetta and J C Lagarias, "Genetic engineering of phytochrome biosynthesis in bacteria." Proc Natl Acad Sci U S A 98(19):10566-71 (2001) PMID 11553807