Coding
FabH2
Part:BBa_K590034
Designed by: Matthew Harger Group: iGEM11_Washington (2011-09-15)
FabH2
This part causes E. coli to produce branched chain fatty acids.
Usage and Biology
FabH2 is from Bacillus subtilis. The FabH family of proteins initiates fatty acid elongation by converting an Acyl-CoA into an Acyl-ACP, with is extended by 2 carbon units to form longer chain length fatty acids. Normally, FabH proteins use a simple 2-carbon acetyl-CoA to start fatty acid biosynthesis, resulting in linear fatty acids. However, FabH2 can also use Isobutyryl-CoA, Isovaleryl-CoA, and 2-Methylbutyryl-CoA (products from Valine, Leucine, and Isoleucine degredation, resulting in 2-methyl branched fatty acid production.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 310
Illegal AgeI site found at 949 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 355
[edit]
Categories
Parameters
None |