Composite
Ptet-ChiX

Part:BBa_K564014

Designed by: Anna Lewinska   Group: iGEM11_DTU-Denmark   (2011-09-20)
Revision as of 23:44, 20 September 2011 by Alewinska88 (Talk | contribs)

atC-->chiX

This device is based on gene encoding small RNA (sRNA), which represses ChiP gene synthesis by pairing with a sequence in 5’ untranslated region (UTR) near ribosome binding site (RBS) of chiP mRNA. This leads to degradation of the targeted mRNA by enzymes called RNases.The repression occurs in the absence of the substrates (chitooligosaccharides) stimulating chiP gene transcription. It is fused with Ptet promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 848
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 838
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None