Part:BBa_K590018

mamMNOPA in vector pGA3K3

This part is made by [http://2011.igem.org/Team:Washington UW iGEM Team 2011]. It includes 5 genes that were found essential for magnetosome formation in Magnetospirillum magneticum strain AMB-1 on a pGA3k3 plasmid backbone.

Usage and Biology

Made by Gibson Cloning method instead of Standard Biobrick method, this part is in a low copy backbone and it belongs to the [http://2011.igem.org/Team:Washington/Magnetosomes/Magnet_Toolkit Magnetosome Toolkit], known as the "middle part of the full assembly".

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 288
  Illegal PstI site found at 74
  Illegal PstI site found at 4039
  Illegal PstI site found at 4757
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 288
  Illegal NheI site found at 1331
  Illegal PstI site found at 74
  Illegal PstI site found at 4039
  Illegal PstI site found at 4757
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 288
  Illegal BglII site found at 361
  Illegal BamHI site found at 1628
  Illegal BamHI site found at 2148
  Illegal BamHI site found at 4144
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 288
  Illegal PstI site found at 74
  Illegal PstI site found at 4039
  Illegal PstI site found at 4757
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 288
  Illegal PstI site found at 74
  Illegal PstI site found at 4039
  Illegal PstI site found at 4757
  Illegal NgoMIV site found at 3180
  Illegal NgoMIV site found at 3553
  Illegal NgoMIV site found at 3709
  Illegal NgoMIV site found at 3832
  Illegal NgoMIV site found at 4627
  Illegal NgoMIV site found at 4814
  Illegal NgoMIV site found at 5851
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 477
  Illegal BsaI.rc site found at 2431
  Illegal SapI.rc site found at 56