Coding

Part:BBa_K613021:Design

Designed by: Nadine Guenat   Group: iGEM11_EPF-Lausanne   (2011-09-20)
Revision as of 01:05, 22 September 2011 by Nguenat (Talk | contribs) (→‎Design Notes)

TetR PQ39YM42LP52 mutant


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The mutation was introduced by site-directed mutagenesis, based on data from [http://www.sciencedirect.com/science/article/pii/S0022283697915400 Helbl and Hillen, 1998].

Source

The TetR gene was PCR-amplified from the Repressilator plasmid. We then introduced mutations by site-directed mutagenesis. Parts of this mutation had already been described by Helbl and Hillen (1998).

References