Coding
Part:BBa_K613018:Design
Designed by: Lilia Salimova Group: iGEM11_EPF-Lausanne (2011-09-20)
TetR Y42F K108E mutant
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The mutation was introduced by site-directed mutagenesis, based on data form [http://www.sciencedirect.com/science/article/pii/S0378111907004623 Krueger et al, 2007]
Source
The TetR gene was PCR-amplified from the Repressilator plasmid. We then introduced the Y42F mutation by site-directed mutagenesis. This mutation had already been described by Krueger et al (2007).