Coding
cspB

Part:BBa_K525223:Design

Designed by: Anna Drong   Group: iGEM11_Bielefeld-Germany   (2011-09-12)
Revision as of 20:43, 20 September 2011 by TimoW (Talk | contribs) (→‎Source)

S-layer cspB from Corynebacterium halotolerans with lipid anchor, PT7 and RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1102
    Illegal XhoI site found at 558
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 225
    Illegal NgoMIV site found at 1314
    Illegal NgoMIV site found at 1425
    Illegal AgeI site found at 216
    Illegal AgeI site found at 457
    Illegal AgeI site found at 504
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 906
    Illegal BsaI.rc site found at 213
    Illegal BsaI.rc site found at 591
    Illegal BsaI.rc site found at 993


Design Notes

This is the cspB S-layer sequence without TAT sequence and with lipid anchor.

Source

  • PCR on the Corynebacterium halotolerans DNA cloned into BioBrick Vectors
    • Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix in the rev primer.
      • fwd: GAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGAAAGAGGAGAAAATGCAGGACGCCCAGGTTGCCAC
      • rev: CTGCAGCGGCCGCTACTAGTATTAGATGCCCAGGCCACCGACG

References