Part:BBa_K590017

mamHIEJKL in vector pGA3K3

This part is made by [http://2011.igem.org/Team:Washington UW iGEM Team 2011]. It includes 6 genes that were found essential for magnetosome formation in Magnetospirillum magneticum strain AMB-1 on a pGA3k3 plasmid backbone.

Usage and Biology

Made by Gibson Cloning method instead of Standard Biobrick method, it belongs to the [http://2011.igem.org/Team:Washington/Magnetosomes/Magnet_Toolkit Magnetosome Toolkit]. Although the sequence was partially verified, chain formation was observed in microscopy.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 5175
  Illegal PstI site found at 8836
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 5175
  Illegal PstI site found at 8836
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 8540
  Illegal XhoI site found at 89
  Illegal XhoI site found at 6375
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 5175
  Illegal PstI site found at 8836
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 5175
  Illegal PstI site found at 8836
  Illegal NgoMIV site found at 238
  Illegal NgoMIV site found at 1254
  Illegal NgoMIV site found at 2589
  Illegal NgoMIV site found at 3920
  Illegal NgoMIV site found at 6754
  Illegal NgoMIV site found at 8562
  Illegal NgoMIV site found at 8929
  Illegal AgeI site found at 5849
  Illegal AgeI site found at 6264
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 651
  Illegal BsaI site found at 1986
  Illegal BsaI site found at 3317
  Illegal BsaI.rc site found at 6947
  Illegal SapI site found at 825
  Illegal SapI site found at 2160
  Illegal SapI site found at 3491
  Illegal SapI.rc site found at 9208
  Illegal SapI.rc site found at 9529