Coding
cjBlue
Part:BBa_K592011
Designed by: Antonio Ascue Avalos Group: iGEM11_Uppsala-Sweden (2011-09-18)
cjBlue, green chromoprotein
This chromoprotein from the Cnidopus japonicus sea anemone, cjBlue, naturally exhibits dark green color when expressed. Compared to amilCP (BBa_K592009) and amilGFP (BBa_K592010), the color development is slower. On agar plates and in liquid culture, the color is readily visible to naked eye after 24-48 hours of incubation. The sequence is codon optimized for expression in E coli.
Usage and Biology
This part is useful as a reporter.
iGEM11_Uppsala-Sweden: Expression of green chromoprotein. Escherichia coli constitutively expressing cjBlue (BBa_K592011).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/reporter/chromoprotein
//collections/chromoprotein/uppsala
//function/reporter/pigment
//collections/chromoprotein/uppsala
//function/reporter/pigment
color | Blue |
protein |