Generator

Part:BBa_K620002

Designed by: Caltech iGEM 2011   Group: iGEM11_Caltech   (2011-09-20)
Revision as of 08:04, 28 September 2011 by Ashelton (Talk | contribs)

LacI promoter + BisdA

Allows for inducible expression of the protein BisdA, one of a pair of proteins that can degrade bisphenol A (BPA). It is controlled by the lacI promoter, which can be induced by IPTG.

Usage and Biology

Plasmid map of K620002 in pSB4A5


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 216
    Illegal AgeI site found at 534
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 407
    Illegal BsaI site found at 443


[edit]
Categories
Parameters
None