Generator
LacI:CspA

Part:BBa_K538203

Designed by: Bas Stringer   Group: iGEM11_Amsterdam   (2011-09-08)
Revision as of 15:35, 19 September 2011 by B.Stringer (Talk | contribs)

pLacI + RBS + CspA

LacI:CspA
K538203


This part was submitted by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] as part of their project: icE. coli. Its purpose is to express the CspA protein from Polaribacter irgensii (BBa_K538003) under control of the Lac operon's promoter, pLacI (BBa_R0010). E. coli expressing this protein experience a drastic increase in their ability to survive freezing and thawing. This Brick's designers intend to use it to culture 'normal' E. coli faster at temperatures below its optimum (37°C), possibly even facilitating growth below the minimal growth temperature of 7°C.


Refer to the main pages of the coding region (CspA: BBa_K538003) for more information on how CspA facilitates cold resistance in E. coli.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
//classic/generator/prc
//function/regulation/transcriptional
//regulation/negative
Parameters
functionProtein expression
negative_regulatorsLacI
outputCspA