Coding
mamSTU

Part:BBa_K590008

Designed by: Michael Brasino, Alicia Wong, Rashmi Ravichandran   Group: iGEM11_Washington   (2011-09-15)
Revision as of 06:09, 22 September 2011 by Saushun (Talk | contribs) (→‎Usage and Biology)

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mamSTU, from Magnetospirillum magneticum

This part includes genes mamS, mamT, and mamU, which were extracted from the genome of Magnetospirillum magneticum strain AMB-1 by [http://2011.igem.org/Team:Washington UW iGEM Team 2011].

Usage and Biology

mamS, mamT, and mamU are AMB0975, AMB0976, and AMB0977 respectively. Specific functions of mamS and mamU were not found. mamT was reported to be related to magnetite crystal growth; participates in different steps during magnetite synthesis.

Below is the gel image of the mamSTU (2030bp):

1kb Ladder (left), mamSTU (right)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1667
    Illegal PstI site found at 437
    Illegal PstI site found at 1949
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1667
    Illegal PstI site found at 437
    Illegal PstI site found at 1949
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1667
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1667
    Illegal PstI site found at 437
    Illegal PstI site found at 1949
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1667
    Illegal PstI site found at 437
    Illegal PstI site found at 1949
    Illegal NgoMIV site found at 1005
    Illegal NgoMIV site found at 1109
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2044


[edit]
Categories
Parameters
n/amamSTU, from Magnetospirillum magneticum