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Part:BBa_K649202

Designed by: SeoHyun Kim   Group: iGEM11_Tokyo_Tech   (2011-09-18)
Revision as of 10:31, 3 October 2011 by Carakim01 (Talk | contribs)

PlacIQ-lox71-rfp-lox66-gfp

The lox sequences, lox71 and lox66, have 5 bp on the 5� and 3� ends changed, respectively. DNA segment flanked by lox71 and lox66 marks the point which the enzyme Cre will excise. The Cre-mediated recombination of this BioBrick had been studied and proved to be work.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1016
    Illegal BamHI site found at 14
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 686
    Illegal AgeI site found at 798
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1700


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Categories
Parameters
n/aPlacIQ-lox71-rfp-lox66-gfp