Composite

Part:BBa_K611014

Designed by: Nicholas Csicsery   Group: iGEM11_UC_Davis   (2011-09-18)
Revision as of 01:29, 25 September 2011 by NCsicsery (Talk | contribs)

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cI Repressible Promoter Characterization Construct

This part is used to characterize mutants of BBa_R0051 (c1 repressible promoter). A mutant of BBa_R0051 can be ligated before this part (on the 5' end) by cutting this plasmid at EcoRI and XbaI and the mutant promoter at EcoRI and SpeI. Several of the mutant's properties can then be measured through the expression of GFP. Certain characteristics of the mutant, such as the binding of the c1 repressor, can be measured after inducing the pBad promoter with arabinose. Concentration of the repressor can be varied through different arabinose concentrations as long as AraC is present.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1008
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 948
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 662


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