Composite

Part:BBa_K523015

Designed by: Edinburgh 2008, 2011   Group: iGEM11_Edinburgh   (2011-09-12)
Revision as of 02:04, 17 October 2019 by Still Water (Talk | contribs)

Plac + RBS + cenA (endoglucanase)

This is C. fimi endoglucanase under the control of the Lac promoter. This was made by Edinburgh 2008 but vector-swapped into pSB1C3 and entered into the Registry by Edinburgh 2011.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 658
    Illegal NotI site found at 1802
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1715
    Illegal BamHI site found at 851
    Illegal XhoI site found at 1213
    Illegal XhoI site found at 1462
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 993
    Illegal NgoMIV site found at 1918
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 897



<ECUST_China> cenA

[edit]
Categories
Parameters
None