Translational_Unit
RBS-GFP-T7

Part:BBa_K606029

Designed by: Axel Séguret, Cyrille Pauthenier   Group: iGEM11_Paris_Bettencourt   (2011-09-18)
Revision as of 16:18, 22 September 2011 by Cyrpaut (Talk | contribs)

RBS SpoVG GFPmut3b T7terminator

This part is a GFP reporter system for B. Subtilis, but that can work in E. coli. The promoter cloned downstream is the one of the T7 RNA polymerase. This stop can also work work standard RNA polymerases.


Usage and Biology

This part has been design to be under the control of a T7 promoter and polymerased by the T7 RNA polymerase for which it has been characterizer. However, we also used it sucessfully in E. coli with a standard promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 886
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 662


[edit]
Categories
//classic/reporter
Parameters
None