Composite

Part:BBa_K537016

Designed by: Ezio Fok   Group: iGEM11_WITS-CSIR_SA   (2011-09-16)
Revision as of 18:15, 19 September 2011 by Eziofok (Talk | contribs)

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Promoter-RBS-CheZ-mCherry-Double terminator

This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by an RBS which is fused to CheZ and monomeric Red fluorescent reporter protein (mRFP) without stop codons in between. The Freiberg iGEM 2007 BioBrick 3.0 fusion protein assembly was used to construct this BioBrick. The RBS-CheZ fusion was constructed via PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015).

Note: All scar sites have been excluded in the annotations. There is a scar site between the Promoter:CheZ-mRFP fusion and CheZ-mRFP fusion:Double terminator. The scar sequence is as follows: ACTAGA

A Freiburg fusion scar site exists between the CheZ and mRFP sequence. The sequence is as follows: ACCGGC

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1245
    Illegal AgeI site found at 1357
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None