Intermediate

Part:BBa_K607015

Designed by: Olesja Popow   Group: iGEM11_Groningen   (2011-09-18)
Revision as of 19:58, 21 September 2011 by JoyceM1013 (Talk | contribs)

cI

cI without degradation tag The cI gene codes for the bacteriophage λ repressor(1). This bacteriophage λ-repressor from phage lambda does not contain a ssrA tag. The ssrA tag is a target for proteolytic enzymes and causes degradation of the protein(2). When this gene is transcribed, it will not be degraded that fast by proteolytic enzymes ( because it lacks the ssrA tag) and so the protein half-life will be longer compared to cI with a degradation tag.

References: (1)Robert T. Sauer* and Robert Anderegg. Primary Structure of the λ Repressor. Biochemistry, 1978. (2) Jeff Hasty, David McMillen, Farren Isaacs and James J. Collins. Computational studies of gene regulatory networks: in numero molecular biology. Nature 268


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None