Coding
cph8

Part:BBa_K592000:Design

Designed by: Lei Sun   Group: iGEM11_Uppsala-Sweden   (2011-09-18)
Revision as of 15:20, 18 September 2011 by SunLei (Talk | contribs) (→‎References)

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cph8 (Cph1/EnvZ fusion)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 364
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The source DNA (pJT122) contained illegal restriction site (PstI) that had to be removed.


Source

Jeffrey Tabor's pJT122 plasmid

References

Tabor, J. J., Levskaya, A., and Voigt, C. A. (2011). Multichromatic control of gene expression in Escherichia coli. J. Mol. Biol. 405, 2, 315–324.