Regulatory

Part:BBa_K553002:Design

Designed by: Giulio Bernardinelli   Group: iGEM11_UNITS_Trieste   (2011-09-17)
Revision as of 13:59, 18 September 2011 by Giulio (Talk | contribs) (→‎Source)

PTraI - TraR-OC8 HLA complex regulated promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 105
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the pcr amplification worked well, few mutation observed after sequencing that are present in our strain

PromTraFw

5' GTTTCTCGAATTCGCGGCCGCTTCTAGAGAAGTGCAGATTTGCACATGAAATCAACG 3'

PromTra rev""

5' GTTTCTTCCTGCAGCGGCCGCTACTAGTATAGCGGAGAGAAAACATCTCATTCAGG 3'

pcr protocol:

95° 5' | 10x (93° 30" | 56° 30" | 72° 40") | 23x ( (93° 30" | 65° 30" | 72° 40") | 72° 7' | 4° ∞


Source

http://www.ncbi.nlm.nih.gov/nucleotide/8572673?report=genbank&log$=nuclalign&blast_rank=1&RID=795BNUZN014

Our A. Tumefaciens strain contains mutations that don't affect the functionality of the promoter

References