Generator
atRS-RFP

Part:BBa_K537008:Design

Designed by: Alexandra Reznichenko   Group: iGEM11_WITS-CSIR_SA   (2011-09-12)
Revision as of 13:36, 18 September 2011 by Sashrez (Talk | contribs) (→‎Design Notes)

Atrazine Riboswitch-mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 9
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 640
    Illegal AgeI site found at 752
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This biobrick part represents a composite of a atrazine riboswitch upstream of a mRFP1 fluorescent protein reporter (derived from part BBa_E1010).

Previous teams: Lethbridge 2007, Lethbridge 2009, and NYMU Taipei 2010 all considered strategies for making the riboswitch a separate biobrick, however standard assembly techniques are inadequate since the sequence distance between the ATG, RBS and aptamer domain of the riboswitch are critical. We have explored this further in a more elaborate discussion here.

Source

The mRFP1 fluorescent reporter is derived from part BBa_E1010

References