Coding

Part:BBa_K676006:Design

Designed by: Ejaj Intisar   Group: iGEM11_UCL_London   (2011-09-17)
Revision as of 02:11, 22 September 2011 by Meng (Talk | contribs) (→‎Design Notes)

T5 Phage Exonuclease


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 600
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Design primers for the PCR to extract the DNA sequence as well as primers for the site directed mutagenesis to remove the XbaI restriction site at the 241th bp position in the 892bp T5 exonuclease DNA sequence.

Source

T5 Bacteriophage

References