Coding
ADC

Part:BBa_K590031

Designed by: Lei Zheng   Group: iGEM11_Washington   (2011-09-15)
Revision as of 18:48, 16 September 2011 by Anjuhye (Talk | contribs)

Aldehyde Decarbonylase (ADC) - Synechococcus elongatus PCC7942

This part encodes an enzyme that removes the carbonyl group (C=O) from a fatty aldehyde, yielding an alkane one carbon shorter than the original aldehyde and a molecule of formate. Works together with Acyl-ACP Reductase to produce odd chain length alkanes.

Fatty Acid Biosynthesis.jpg

Usage and Biology

When AAR and ADC are co-expressed, they produce odd chain length alkanes from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team].

GC plot showing that alkanes are only produced when both ADC and AAR are expressed.
GC-MS quantification shows greater alkane production under UW iGEM 2011's "optimized conditions" (see wiki for details)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 730
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None