Measurement

Part:BBa_K546002

Designed by: M. Hesselman   Group: iGEM11_Wageningen_UR   (2011-09-14)
Revision as of 18:57, 21 September 2011 by RubenvanHeck (Talk | contribs)

Lux pL controlled luxR with lux pR controlled GFP(lva tag).

This device consists of two protein generators. The first generator ‘luxpL-RBS-C0062-B0015’ produces an intermediate level of the LuxR protein. The second generator ‘luxpR-RBS-J04031-B0015’has a low basal expression of the GFP, which can - of course - be monitored. 

It should be noted that if AHL is added to this system it forms a complex with LuxR which will increase transcription of GFP while reducing the transcription of LuxR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101
    Illegal BsaI.rc site found at 1828


[edit]
Categories
//classic/signalling/receiver
//function/cellsignalling
Parameters
control(1) R0063 & (2) R0062
device_typefeedback
directionForward
latencySeconds
protein(1) LuxR & (2) GFP-LVA
signalling_molecule3OC6HSL (AHL)
switch_point(1) 2nM