Coding
mamKL

Part:BBa_K590003

Designed by: Michael Brasino, Alicia Wong, Rashmi Ravichandran   Group: iGEM11_Washington   (2011-09-15)
Revision as of 05:52, 22 September 2011 by Saushun (Talk | contribs) (→‎Usage and Biology)

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mamKL, from Magnetospirillum magneticum

This part includes genes mamK and mamL, which were extracted from the genome of Magnetospirillum magneticum strain AMB-1 by [http://2011.igem.org/Team:Washington UW iGEM Team 2011].

Usage and Biology

mamKL is 1336bp in length and the gene numbers are AMB0965 and AMB0966 respectively; it was reported that mamK is required for proper magnetosome chain organization; it is bacterial actin-like cytoskeleton protein required for proper alignment of the magnetosomes in a chain, shown to localize the mamI; mamL is crucial to mangneosome membrane creation, shown to be spread across the cell membrane and sometimes forms lines.

Below is the gel image of mamKL:

1kb Ladder (right), mamKL (left)


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 666
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 666
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 370
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 666
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 666
    Illegal NgoMIV site found at 392
    Illegal NgoMIV site found at 759
    Illegal NgoMIV site found at 906
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1038
    Illegal SapI.rc site found at 1359


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Categories
Parameters
n/amamKL, from Magnetospirillum magneticum