Coding
AAR

Part:BBa_K590032

Designed by: Lei Zheng   Group: iGEM11_Washington   (2011-09-15)
Revision as of 02:50, 16 September 2011 by Swanson (Talk | contribs) (→‎Usage and Biology)

Acyl-ACP Reductase (AAR) - Synechococcus elongatus PCC7942

This part encodes an enzyme that reduces cellular fatty acyl-ACPs, from the bacterial fatty acid biosynthesis pathway, into fatty aldehydes. Use together with Aldehyde Decarbonylase (ADC) to generate alkanes from acyl-ACPs. This part has been codon-optimized for E. coli.

Fatty Acid Biosynthesis.jpg

Usage and Biology

The [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team] worked out conditions to grow E. coli containing AAR and ADC to generate alkanes.


Alkanes Final Picture.jpg
GC plot showing that alkanes are only produced when both ADC and AAR are expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1060
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None