Device

Part:BBa_K540000

Designed by: Melanie Geffroy, Philippe Thomas   Group: iGEM11_Lyon-INSA-ENS   (2011-09-15)
Revision as of 20:55, 17 September 2011 by Alex273 (Talk | contribs)

rcn-csgBAEFG, over-induces adherence in response to cobalt

This parts associates the rcn promoter, whose expression increases with cobalt in the medium, with csgBAEFG, an operon that enables the production and secretion of curlis, which are adherent proteins. It contains the necessary RBS to work. With this part, strains can become adherent in response to cobalt in the medium. This part may also make bacteria adherent in response to nickel, which has however not been studied.

Safety

This part is not toxic by itself. However, when using this part, you will probably need to handle cobalt. Cobalt is toxic in all its forms ( ionic or metallic ) by inhalation, ingestion or contact. Wear adapted personal protection equipment ( labcoat, safety glasses, safety gloves ) and dispose of it in appropriate waste containers.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 47
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2108
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None