Coding
Part:BBa_K515007
Designed by: Atipat Patharagulpong Group: iGEM11_Imperial_College_London (2011-09-12)
Dendra2
Description
This part consists of coding sequence codon optimised for Escherichia coli & Bacillus subtilis. It is part of the composite biobrick BBa_K515107. This sequence codes for photoconvertible protein Dendra, capable of being irreversibly photoconverted by single-photon stimulation from excitation at 486nm and emission at 505nm wavelength to 558nm excitation and 575nm emission wavelength. Photoconversion can occur using two wavelengths 488 and 405nm (Gurskaya et al., 2006).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds
chassis | E. coli DH5α |
device_type | Fluorescent reporter |
emission | 505 nm and 575 nm |
excitation | 486 nm and 558 nm |
protein | K515007 |
resistance | Chloramphenicol |