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Composite

Part:BBa_K515107

Designed by: Atipat Patharagulpong   Group: iGEM11_Imperial_College_London   (2011-09-12)
Revision as of 22:25, 15 September 2011 by Nk2909 (Talk | contribs)

Composite p(tetR) - Dendra2

Description

Coding sequence BBa_K515007, under the control of repressible promoter TetR BBa_R0040 with RBS BBa_B0034. Coding sequence BBa_K515007 codes for Dendra a photoconvertible reporter protein. In its natural state, it is excited and emits at 486 and 505nm, respectively. This appears green and therefore in its natural state Dendra can be used as GFP reporter. However it can be irreversibly converted to be excited at 558nm and consequently emit at 575nm by single-photon stimulation. After conversion reporter appears red and can be observed as RFP. Conversion can be efficiently achieved at wavelengths of both 488 and 405nm (Gurskaya et al., 2006). However, the cytotoxic wavelength of 405 converts Dendra more efficiently (data not shown).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/reporter/multiple
Parameters
chassisE. coli DH5α
controlR0040
device_typeFluorescent reporter
emission505 nm and 575 nm
excitation486 nm and 558 nm
proteinK515007
rbsB0034
resistanceChloramphenicol